Healthcare is changing, and it's critical to take use of new technology to generate fresh data and assist the adoption of precision medicine (PM). Biomarkers are increasingly being used in clinical trials to enhance patient outcomes, and they are quickly becoming ubiquitous in clinical practice. A diagnostic biomarker is a biological parameter that can be used to help diagnose a disease and may also be used to predict disease progression and treatment success. A laboratory, radiological, genetic, anatomical, physiological, or other finding that aids in the differentiation of one disease from another. The pharmaceutical industry's high failure rate has pushed biomarkers and personalized medicine to the forefront as potential answers. Biomarkers and companion diagnostics (CDx) have the potential to help the pharmaceutical business increase the likelihood of success, shorten time to market, and, most significantly, improve patients by enabling accurate diagnosis and selection of the most effective and least toxic therapeutics.